資源簡介
SIM顯微鏡重建原理的matlab源文件
代碼片段和文件信息
%a?program?for?simple?SIM
clear?all
close?all;
%initial?image
im=imread(‘E:\gradu\lena.png‘);
inm=im(1+100:256+1001+100:256+100);
inm=im2double(inm);
finm=fft2(inm);
finm=fftshift(finm);
%?finm=ifft2(finm);
afinm=abs(finm);
%?figure;
%?imshow(afinm);
%?imshow(afinm/max(max(afinm)));
%blurred?image
psf=fspecial(‘gaussian‘[256256]3);
bim=imfilter(inmpsf‘same‘);???%blurred?image
figure;
imshow(bim);
otf=fft2(psf);
otf=fftshift(otf);
otf=abs(otf);
%?figure;
%?imshow(otf);
fbim=fft2(bim);
fbim=fftshift(fbim);
afbim=abs(fbim);?%the?abs?of?fbim
%?figure;
%?imshow(afbim);
%?imshow(afbim/max(max(afbim)));
%?structured?illumination
k0=1;
fi=0;
?for?ii=1:256
?????for?jj=1:256
?????????i1(iijj)=1+0.5*cos(k0*jj+fi);
?????????i2(iijj)=1+0.5*cos(k0*jj+1/3*pi);
?????????i3(iijj)=1+0.5*cos(k0*jj+2/3*pi);
?????end
?end
%??fi=fft2(i1);
%??fi=fftshift(fi);
%??afi=abs(fi);
%??
%??figure;
%??imshow(afi/max(max(afi)))
%??figure;
%??imshow(i1);
%??figure;
%??imshow(i2);
%??figure;
%??imshow(i3);
%detected?images?under?structured?illumination
d1=imfilter(inm.*i1psf‘same‘);
d2=imfilter(inm.*i2psf‘same‘);
d3=imfilter(inm.*i3psf‘same‘);
%?figure;
%?imshow(d1);
%?figure;
%?imshow(d2);
%?figure;
%?imshow(d3);
%reconstruction
fd1=fft2(d1);
fd2=fft2(d2);
fd3=fft2(d3);
fd1=fftshift(fd1);
fd2=fftshift(fd2);
fd3=fftshift(fd3);
%?afd1=abs(fd1);
%?afd2=abs(fd2);
%?afd3=abs(fd3);
%?figure;
%?imshow(afd1);
%?title(‘fourier?detection1‘);
%?figure;
%?imshow(afd2);
%?title(‘fourier?detection2‘);
%?figure;
%?imshow(afd3);
%?title(‘fourier?detection3‘);
m=[10.50.5;10.5*exp(i*pi/3)0.5*exp(-i*pi/3);10.5*exp(i*pi*2/3)0.5*exp(-i*pi*2/3)];
m1=inv(m);
for?ii=1:256
????for?jj=1:256
??????s11(iijj)=m1(1:)*([fd1(iijj)fd2(iijj)fd3(iijj)]‘);
??????s12(iijj)=m1(2:)*([fd1(iijj)fd2(iijj)fd3(iijj)]‘);
??????s13(iijj)=m1(3:)*([fd1(iijj)fd2(iijj)fd3(iijj)]‘);
????end
end
figure;
imshow(abs(s11));
figure;
imshow(abs(s12));
figure;
imshow(abs(s13));
%2nd?direction
%?structured?il
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